Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

Mfr.No.

EK0592

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.027	0.069	0.101	0.173	0.323	0.577	1.092	1.856

Description

Rat IL-18 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif;

Molecular Weight

22303 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H37-S194

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat IL-18

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	29	166	511	29	165	490
Standard deviation	1.97	2.2	22.99	2.2	9.07	29.4
CV (%)	6.8%	4.7%	4.5%	7.6%	5.5%	6%

Protein Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Background

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	29	29	30	28	29	0.7	2.4%
Sample 2	166	149	165	152	158	7.58	4.7%
Sample 3	511	489	520	499	504	11.75	2.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29197
Antigen	IL-18/IL-1F4/Interleukin-18
Gene Name	IL18
UniProt ID	P97636
Protein Name	Interleukin-18

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cancer Metabolism,Cytokines,Immunology,Innate Immunity,Interleukins,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000537	EK0592	96 wells/kit	$399		Add to Cart

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

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