Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Mfr.No.

EK1301

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.024	0.067	0.178	0.301	0.463	0.761	1.345	1.860

Description

Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine® (96 Tests). Quantitate Rat Hgf in cell culture supeatants, serum and plasma (EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatocyte growth factor;Hepatopoietin-A;Scatter factor;SF;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;Hgf;

Molecular Weight

82906 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q33-R495 (alpha) & V496-L728 (beta)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat HGF

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	223	1031	4762	201	1018	4682
Standard deviation	12.48	78.35	371.43	12.46	90.6	412.01
CV (%)	7.6%	7.6%	7.8%	6.2%	8.9%	8.8%

Protein Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). .

Background

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	223	205	208	224	215	8.57	3.9%
Sample 2	1031	1143	985	1076	1058	58.31	5.5%
Sample 3	4762	4701	5150	5432	5011	297.76	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24446
Gene Name	HGF
UniProt ID	P17945
Protein Name	Hepatocyte growth factor
Sequence	Belongs to the peptidase S1 family. Plasminogen subfamily.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Growth Factors/Hormones,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000893	EK1301	96 wells/kit	$499		Add to Cart

Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine®

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