Rat Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

Mfr.No.

EK1371

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.019	0.06	0.092	0.178	0.406	0.799	1.611	2.283

Description

Rat Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine® (96 Tests). Quantitate Rat Hjv in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hemojuvelin;Hemochromatosis type 2 protein homolog;RGM domain family member C;Hfe2;Rgmc;

Molecular Weight

45221 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q36-D396

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Hemojuvelin

Subcellular Localization

Cell membrane ; Lipid-anchor, GPI-anchor . Also released in the extracellular space. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	180	515	1176	180	499	1182
Standard deviation	10.44	29.87	56.44	11.34	34.93	74.46
CV (%)	5.8%	5.8%	4.8%	7%%	7%	6.3%

Protein Function

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .

Background

Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alteatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	180	150	175	152	164	13.38	8.1%
Sample 2	515	494	479	531	504	19.82	3.9%
Sample 3	1176	1181	1090	1127	1143	37.4	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	310681
Gene Name	Hjv
UniProt ID	Q8N7M5
Protein Name	Hemojuvelin

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001126	EK1371	96 wells/kit	$499		Add to Cart

Rat Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

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