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Home / ELISA Kit

Rat Growth Factor Receptor-Bound Protein 2 - GRB2 ELISA Kit

SKU : BHE12113195

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

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Bioassay Technology Laboratory

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Mfr.No.

E1996Ra

Sensitivity

3.24ng/L

Detection Range

6-1800ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Adapter protein GRB2;GRB 2;GRB2;Growth factor receptor-bound protein 2;Protein Ash;SH2/SH3 adapter GRB2

Molecular Weight

25206

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1996Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	329.3	15.76	4.8
Intra-Assay	2	18	453.8	15.68	3.5
Intra-Assay	3	18	126	7.74	6.1

Background

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

Protocol

These standard curves of E1996Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.195	2.155	2.101
	2.115
480ng/L	1.391	1.34	1.286
	1.289
240ng/L	0.735	0.729	0.675
	0.723
120ng/L	0.531	0.518	0.464
	0.505
60ng/L	0.289	0.306	0.252
	0.323
0ng/L	0.048	0.054	0
	0.06

Gene ID	81504
UniProt ID	P62994

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113195	E1996Ra	48T	$320		Add to Cart
BHE12113195	E1996Ra	96T	$458		Add to Cart

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