Rat Glycine N-methyltransferase

Mfr.No.

E1991Ra

Alternative Names

Folate-binding protein;Glycine N-methyltransferase;GNMT

Reactivity

Rat

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32549

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.85ng/L

Background

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Gene ID	25134
UniProt ID	P13255
Antigen	GNMT

Research Area

Signal Transduction

Protocol

These standard curves of E1991Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.237	2.33	2.288
	2.422
1200ng/L	1.383	1.284	1.243
	1.185
600ng/L	0.645	0.676	0.634
	0.706
300ng/L	0.325	0.322	0.28
	0.318
150ng/L	0.21	0.171	0.129
	0.132
0ng/L	0.052	0.041	0
	0.031

Precision

We measured random samples of E1991Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1329.1	38.24	2.9
Intra-Assay	2	18	772.6	40.24	5.2
Intra-Assay	3	18	323.6	16.5	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113190	E1991Ra	48T	$320		Add to Cart
BHE12113190	E1991Ra	96T	$458		Add to Cart

Rat Glycine N-methyltransferase - GNMT ELISA Kit

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