Rat Glucose Transporter 1 - GLUT1 ELISA Kit

Mfr.No.

E1087Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Solute Carrier Family 2, Facilitated Glucose Transporter Member (also known as SLC2A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

High affinity sodium-glucose cotransporter;Na(+/glucose cotransporter 1;SLC 5A1;SLC5A1;Sodium/glucose cotransporter 1;Solute carrier family 5 member 1

Reactivity

Rat

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

73066

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.103ng/ml

Background

Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

Gene ID	25552
UniProt ID	P53790
Antigen	SLC5A1

Research Area

Signal Transduction

Protocol

These standard curves of E1087Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.177	2.267	2.204
	2.356
16ng/ml	1.558	1.523	1.46
	1.488
8ng/ml	0.949	0.95	0.887
	0.95
4ng/ml	0.498	0.509	0.446
	0.519
2ng/ml	0.301	0.266	0.204
	0.232
0ng/ml	0.069	0.063	0
	0.057

Precision

We measured random samples of E1087Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6	0.29	4.8
Intra-Assay	2	18	12.2	0.47	3.8
Intra-Assay	3	18	7.4	0.43	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112335	E1087Ra	48T	$320		Add to Cart
BHE12112335	E1087Ra	96T	$458		Add to Cart

Rat Glucose Transporter 1 - GLUT1 ELISA Kit

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