Rat Glial Fibrillary Acidic Protein

Mfr.No.

E0538Ra

Sensitivity

5.43pg/ml

Detection Range

10-2000pg/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of glial fibrillary acidic protein (also known as GFAP ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GFAP;Glial fibrillary acidic protein

Molecular Weight

49957

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0538Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	429.3	20.19	4.7
Intra-Assay	2	18	191.1	9.92	5.2
Intra-Assay	3	18	67	4.14	6.2

Background

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Protocol

These standard curves of E0538Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.213	2.23	2.189
	2.247
600pg/ml	1.711	1.651	1.611
	1.591
300pg/ml	1.191	1.142	1.101
	1.092
150pg/ml	0.667	0.659	0.619
	0.651
75pg/ml	0.472	0.45	0.409
	0.428
0pg/ml	0.031	0.041	0
	0.05

Gene ID	24387
UniProt ID	P47819

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111819	E0538Ra	48T	$320		Add to Cart
BHE12111819	E0538Ra	96T	$458		Add to Cart

Rat Glial Fibrillary Acidic Protein - GFAP ELISA Kit

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