Rat GDF5/Bmp 14 ELISA Kit PicoKine®

Mfr.No.

EK1504

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.082	0.144	0.173	0.283	0.409	0.787	1.385	2.050

Description

Rat GDF5/Bmp 14 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gdf5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth and differentiation factor-5 ;Gdf5 ;gdf-5 ;

Molecular Weight

7491 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A376-R495

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat GDF5

Subcellular Localization

Secreted .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	323	894	59	339	908
Standard deviation	3.84	14.53	50.06	4.72	21.01	57.2
CV (%)	6.4%	4.5%	5.6%	8%	6.2%	6.3%

Background

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	59	68	62	62	3.49	5.6%
Sample 2	323	317	316	281	309	16.52	5.3%
Sample 3	894	859	910	831	873	30.69	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	252835
Antigen	GDF5/Bmp
Gene Name	Gdf5
UniProt ID	Q8K4X5
Protein Name	Growth and differentiation factor-5

Applications	ELISA
Research Area	Metabolism,Mitochondrial,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001093	EK1504	96 wells/kit	$750		Add to Cart

Rat GDF5/Bmp 14 ELISA Kit PicoKine®

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