Rat Gamma Glutamyl Transpeptidase 1

Mfr.No.

E1194Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Gamma-Glutamyl Transferase (also known as GGT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD224;Gamma-glutamyltransferase 1;Gamma-glutamyltranspeptidase 1;GGT 1;GGT1;Glutathione hydrolase 1 heavy chain;Glutathione hydrolase 1 light chain;Glutathione hydrolase 1 proenzyme;Leukotriene-C4 hydrolase;

Reactivity

Rat

Detection Range

2-600mIU/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

61610

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.13mIU/mL

Background

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.

Gene ID	116568
UniProt ID	P07314
Antigen	GGT1

Research Area

Metabolism

Protocol

These standard curves of E1194Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320mIU/mL	2.25	2.208	2.168
	2.166
160mIU/mL	1.623	1.685	1.644
	1.746
80mIU/mL	1.062	1.047	1.006
	1.031
40mIU/mL	0.677	0.696	0.656
	0.716
20mIU/mL	0.496	0.45	0.409
	0.404
0mIU/mL	0.05	0.041	0
	0.031

Precision

We measured random samples of E1194Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	122.5	5.27	4.3
Intra-Assay	2	18	66.6	4.97	7.5
Intra-Assay	3	18	16.6	1.21	7.3

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12112440	E1194Ra	48T	$320		Add to Cart
BHE12112440	E1194Ra	96T	$458		Add to Cart

Rat Gamma Glutamyl Transpeptidase 1 - GGT1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Gamma Glutamyl Transpeptidase 1 - GGT1 ELISA Kit

Rate it now..!!