Rat Fructose-1 - 6-bisphosphatase 1

Mfr.No.

E0977Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Fructose-1, 6-bisphosphatase 1 (also known as FBP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1;FBP 1;FBP1;FBPase 1;Fructose-1,6-bisphosphatase 1;Liver FBPase

Reactivity

Rat

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

39609

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.14ng/L

Background

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Gene ID	24362
UniProt ID	P19112
Antigen	FBP1

Research Area

Others

Protocol

These standard curves of E0977Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.452	2.3	2.233
	2.148
1200ng/L	1.361	1.354	1.287
	1.347
600ng/L	0.7	0.738	0.67
	0.775
300ng/L	0.444	0.442	0.375
	0.441
150ng/L	0.33	0.301	0.233
	0.271
0ng/L	0.06	0.067	0
	0.074

Precision

We measured random samples of E0977Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1650.1	66.29	4
Intra-Assay	2	18	1012.1	54.18	5.4
Intra-Assay	3	18	585.7	42.06	7.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112226	E0977Ra	48T	$320		Add to Cart
BHE12112226	E0977Ra	96T	$458		Add to Cart

Rat Fructose-1 - 6-bisphosphatase 1 - FBP1 ELISA Kit

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