Rat Flt-3 ligand ELISA Kit PicoKine®

Mfr.No.

EK2101

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.030	0.117	0.199	0.323	0.590	0.939	1.654	2.280

Description

Rat Flt-3 ligand ELISA Kit PicoKine® (96 Tests). Quantitate Rat Flt3lg in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fms-related tyrosine kinase 3 ligand; Flt3lg

Immunogen

Expression system for standard: NS0; Immunogen sequence: G27-R188

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Flt-3 ligand

Subcellular Localization

Extracellular space. Intrinsic component of exteal side of plasma membrane. Integral component of membrane.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	160	699	41	184	759
Standard deviation	1.62	6.88	28.66	2.46	10.67	49.34
CV (%)	4.5%	4.3%	4.1%	6.0%	5.8%	6.5%

Background

Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	37	33	34	35	1.58	4.5%
Sample 2	160	162	184	182	172	11.04	6.4%
Sample 3	699	680	621	637	659	31.49	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	103691134
Gene Name	Flt3lg
UniProt ID	A0A1W2Q6J3

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001649	EK2101	96 wells/kit	$499		Add to Cart

Rat Flt-3 ligand ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Flt-3 ligand ELISA Kit PicoKine®

Rate it now..!!