Rat Fibroblast Growth Factor-23

Mfr.No.

E0170Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Fibroblast Growth Factor-23 (also known as FGF-23) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FGF 23;FGF23;FGF-23;Fibroblast growth factor 23

Reactivity

Rat

Detection Range

5-1500pg/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27912

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.56pg/ml

Background

Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL.

Gene ID	170583
UniProt ID	Q8VI82
Antigen	FGF23

Research Area

Signal Transduction

Protocol

These standard curves of E0170Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800pg/ml	2.049	2.044	1.988
	2.038
400pg/ml	1.744	1.848	1.792
	1.951
200pg/ml	1.123	1.147	1.091
	1.17
100pg/ml	0.704	0.711	0.655
	0.718
50pg/ml	0.417	0.417	0.361
	0.416
0pg/ml	0.056	0.056	0
	0.055

Precision

We measured random samples of E0170Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	248	11.23	4.5
Intra-Assay	2	18	179.6	12.43	6.9
Intra-Assay	3	18	52	2.77	5.3

Storage	2-8°C

Reference	Investigating the effect of testosterone by itself and in combination with letrozole on 1, 25-dihydroxy vitamin D and FGF23 in male rats Journal: J Endocrinol Invest. 2019 Jan;42(1):19-25.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111486	E0170Ra	48T	$320		Add to Cart
BHE12111486	E0170Ra	96T	$458		Add to Cart

Rat Fibroblast Growth Factor-23 - FGF-23 ELISA Kit

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