Rat FGFBP1 ELISA Kit PicoKine®

Mfr.No.

EK1695

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	78	156	313	625	1250	2500	5000
O.D.	0.066	0.156	0.211	0.336	0.565	0.991	1.741	2.475

Description

Rat FGFBP1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Fgfbp1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fibroblast growth factor-binding protein 1;FGF-BP;FGF-BP1;FGF-binding protein 1;Growth factor-binding protein 1;FGFBP-1;Fgfbp1

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-C238

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat FGFBP1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	112	566	2662	119	607	2774
Standard deviation	6.9	36.79	114.47	6.19	37.03	199.7
CV (%)	6.2%	6.5%	4.3%	5.2%	6.1%	7.2%

Protein Function

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Background

Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 5.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	112	108	117	112	112	3.19	2.8%
Sample . 2	566	629	674	611	620	38.71	6.2%%
Sample 3	2662	2454	2765	2801	2670	135.00	5.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	64535
Gene Name	Fgfbp1
UniProt ID	Q9QY10
Tissue Specificity	Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001360	EK1695	96 wells/kit	$499		Add to Cart

Rat FGFBP1 ELISA Kit PicoKine®

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