Rat Fcoagulation Factor 9

Mfr.No.

E0493Ra

Sensitivity

1.51ng/mL

Detection Range

3-900ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Fcoagulation Factor IX (also known as FIX) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Christmas factor;Coagulation factor IX;Coagulation factor IXa heavy chain;Coagulation factor IXa light chain;F9

Molecular Weight

51808

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0493Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	215.6	13.37	6.2
Intra-Assay	2	18	346.1	12.58	3.6
Intra-Assay	3	18	79.9	6.28	7.9

Background

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

Protocol

These standard curves of E0493Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/mL	2.111	2.189	2.147
	2.268
240ng/mL	1.29	1.263	1.221
	1.236
120ng/mL	0.811	0.761	0.719
	0.711
60ng/mL	0.526	0.523	0.481
	0.52
30ng/mL	0.219	0.225	0.182
	0.23
0ng/mL	0.025	0.042	0
	0.059

Gene ID	24946
UniProt ID	P16296

Research Area	Cardiovascular; Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111780	E0493Ra	48T	$320		Add to Cart
BHE12111780	E0493Ra	96T	$458		Add to Cart

Rat Fcoagulation Factor 9 - F9 ELISA Kit

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