Rat FABP3 ELISA Kit PicoKine®

Mfr.No.

EK1623

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	13000	3000	6000
O.D.	0.075	0.166	0.229	0.356	0.588	1.016	1.722	2.411

Description

Rat FABP3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Fabp3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-A133

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat FABP3

Subcellular Localization

Cytoplasm.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	131	866	2555	124	868	2555
Standard deviation	6.02	54.55	158.41	7.19	60.76	181.4
CV (%)	4.6%	7%%	6.2%	5.8%	7%	7.1%

Protein Function

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Background

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	131	127	121	131	127	4.09	3.2%
Sample 2	866	923	850	898	884	28.26	3.1%
Sample 3	2555	2241	2468	2352	2404	118.5	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79131
Gene Name	Fabp3
UniProt ID	P07483
Tissue Specificity	Heart, but also skeletal muscle, kidney, brain and mammary gland.

Applications	ELISA
Research Area	Fatty Acids,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Redox Metabolism,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001339	EK1623	96 wells/kit	$499		Add to Cart

Rat FABP3 ELISA Kit PicoKine®

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