Rat F-Box Only Protein 32 - FBXO32 ELISA Kit

Mfr.No.

E1131Ra

Sensitivity

9.89ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Atrogin1;Atrogin-1;F-box only protein 32;FBXO32;MAFbx;Muscle atrophy F-box protein

Molecular Weight

40926

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1131Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1847.97	62.07	3.36
Intra-Assay	2	18	326.95	20.9	6.39
Intra-Assay	3	18	972.63	44.87	4.61

Background

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).

Protocol

These standard curves of E1131Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.217	2.181	2.137
	2.145
1200ng/L	1.126	1.193	1.148
	1.259
600ng/L	0.759	0.77	0.726
	0.782
300ng/L	0.377	0.414	0.37
	0.452
150ng/L	0.254	0.259	0.215
	0.264
0ng/L	0.051	0.044	0
	0.038

Gene ID	171043
UniProt ID	Q91Z62

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112378	E1131Ra	48T	$320		Add to Cart
BHE12112378	E1131Ra	96T	$458		Add to Cart

Rat F-Box Only Protein 32 - FBXO32 ELISA Kit

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