Rat Extracellular Superoxide Dismutase - Cu-Zn

Mfr.No.

E1185Ra

Sensitivity

0.29ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Extracellular Superoxide Dismutase [Cu,Zn] (also known as Cu/Zn-SOD/SOD3) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Cu;EC-SOD;ECSODPT;Extracellular superoxide dismutase (Cu-Zn;Superoxide dismutase B

Molecular Weight

26620

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1185Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.66	0.97	5.47
Intra-Assay	2	18	28.46	0.93	3.26
Intra-Assay	3	18	13.2	0.97	7.38

Background

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

Protocol

These standard curves of E1185Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.152	2.254	2.22
	2.357
32ng/ml	1.401	1.387	1.352
	1.372
16ng/ml	0.847	0.794	0.76
	0.741
8ng/ml	0.447	0.437	0.403
	0.427
4ng/ml	0.249	0.247	0.213
	0.246
0ng/ml	0.036	0.034	0
	0.033

Gene ID	25352
UniProt ID	Q08420

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112431	E1185Ra	48T	$320		Add to Cart
BHE12112431	E1185Ra	96T	$458		Add to Cart

Rat Extracellular Superoxide Dismutase - Cu-Zn - SOD3 ELISA Kit

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