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Rat Estrogen Receptor Beta - ER-beta ELISA Kit

BHE12112368

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Lacks ligand binding affinity and suppresses ESR1/ER-alpha and ESR2 isoform 1/ER-beta1 mediated transcriptional activation and may act as a dominant negative regulator of estrogen action. Unable to bind DNA and activate transcription due to the truncation of the DNA binding domain. Unable to bind DNA and activate transcription due to the truncation of the DNA binding domain.

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Gene ID 25149
UniProt ID Q62986
Antigen ER-beta
Research Area Epigenetics and Nuclear Signaling
Protocol These standard curves of E1121Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.325 2.186 2.136
2.047
800ng/L 1.74 1.822 1.772
1.904
400ng/L 1.287 1.25 1.2
1.214
200ng/L 0.606 0.662 0.612
0.718
100ng/L 0.424 0.401 0.351
0.378
0ng/L 0.064 0.05 0
0.037
Precision We measured random samples of E1121Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 492.4 21.04 4.27
Intra-Assay 2 18 372.11 22.81 6.13
Intra-Assay 3 18 143 7.11 4.98
Storage 2-8°C
SKU Supplier No. Size Your price Quantity
BHE12112368 E1121Ra 48T $320
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BHE12112368 E1121Ra 96T $458
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