Rat EPO/Erythropoietin ELISA Kit PicoKine®

Mfr.No.

EK1351

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.000	0.008	0.016	0.066	0.162	0.449	1.420	2.090

Description

Rat EPO/Erythropoietin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Epo in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Erythropoietin;Epo;

Molecular Weight

21286 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T44-K226

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat EPO

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	108	342	1332	113	322	1373
Standard deviation	5.4	25.39	70.59	6.66	30.26	86.49
CV (%)	5%	7.6%	5.3%	5.9%	9.4%	6.3%

Protein Function

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Background

Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. Whats more, EPO is also involved in the wound healing process.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	108	117	110	115	112	3.64	3.2%
Sample 2	342	374	386	373	368	16.26	4.4%
Sample 3	1332	1189	1227	1391	1284	80.66	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24335
Gene Name	EPO
UniProt ID	P29676
Protein Name	Erythropoietin
Tissue Specificity	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Applications	ELISA
Research Area	Angiogenesis,Apoptosis,Atherosclerosis,Cardiovascular,G Protein Signaling,Hematopoietic Progenitors,Hypertrophy,Immunoglobulins,Immunology,Receptors,Signal Transduction,Signaling Pathway,Small G Proteins,Stem Cells,Surface Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000912	EK1351	96 wells/kit	$499		Add to Cart

Rat EPO/Erythropoietin ELISA Kit PicoKine®

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