Rat Eotaxin 1 - CCL11 ELISA Kit

Mfr.No.

E0558Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 28A (also known as IL-28A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 11;CCL 11;CCL11;Eosinophil chemotactic protein;Eotaxin;Small-inducible cytokine A11

Reactivity

Rat

Detection Range

1-380ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

10851

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.51ng/L

Background

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.

Gene ID	29397
UniProt ID	P97545
Antigen	CCL11

Research Area

Immunology

Protocol

These standard curves of E0558Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200ng/L	2.188	2.194	2.139
	2.199
100ng/L	1.343	1.345	1.291
	1.348
50ng/L	0.93	0.939	0.884
	0.947
25ng/L	0.548	0.513	0.459
	0.479
12.5ng/L	0.305	0.282	0.227
	0.259
0ng/L	0.056	0.055	0
	0.053

Precision

We measured random samples of E0558Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	21.4	1.53	7.1
Intra-Assay	2	18	56.4	2.73	4.8
Intra-Assay	3	18	148.1	6.19	4.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111836	E0558Ra	48T	$320		Add to Cart
BHE12111836	E0558Ra	96T	$458		Add to Cart

Rat Eotaxin 1 - CCL11 ELISA Kit

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