Matrices listed below were spiked with certain level of EL and the recovery rates were calculated by comparing the measured value to the expected amount of EL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
94
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
86-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.