Rat DNA-binding Protein Inhibitor Id-1

Mfr.No.

E1949Ra

Alternative Names

DNA-binding protein inhibitor ID-1;ID 1;ID1;Inhibitor of differentiation 1;Inhibitor of DNA binding 1

Reactivity

Rat

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

17397

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.21ng/L

Background

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Gene ID	25261
UniProt ID	P41135
Antigen	ID1

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E1949Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.513	2.597	2.558
	2.68
600ng/L	1.33	1.351	1.312
	1.372
300ng/L	0.796	0.808	0.769
	0.82
150ng/L	0.449	0.483	0.444
	0.516
75ng/L	0.282	0.256	0.217
	0.23
0ng/L	0.043	0.039	0
	0.035

Precision

We measured random samples of E1949Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	690.6	17.19	2.5
Intra-Assay	2	18	255.4	13.34	5.2
Intra-Assay	3	18	450.1	26.96	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113148	E1949Ra	48T	$320		Add to Cart
BHE12113148	E1949Ra	96T	$458		Add to Cart

Rat DNA-binding Protein Inhibitor Id-1 - ID1 ELISA Kit

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