Rat Dicarbonyl and L-xylulose Reductase

Mfr.No.

E1265Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Dicarbonyl/L-Xylulose Reductase (also known as DCXR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

DCXR;Dicarbonyl/L-xylulose reductase;L-xylulose reductase;XR

Reactivity

Rat

Detection Range

0.2-60ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

25720

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.107ng/ml

Background

Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.

Gene ID	171408
UniProt ID	Q920P0
Antigen	DCXR

Research Area

Others

Protocol

These standard curves of E1265Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.168	2.243	2.179
	2.319
16ng/ml	1.824	1.797	1.732
	1.77
8ng/ml	1.319	1.277	1.212
	1.235
4ng/ml	0.621	0.625	0.561
	0.63
2ng/ml	0.404	0.399	0.334
	0.394
0ng/ml	0.063	0.065	0
	0.067

Precision

We measured random samples of E1265Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.18	5.8
Intra-Assay	2	18	15.9	1.04	6.5
Intra-Assay	3	18	5.3	0.26	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112508	E1265Ra	48T	$320		Add to Cart
BHE12112508	E1265Ra	96T	$458		Add to Cart

Rat Dicarbonyl and L-xylulose Reductase - DCXR ELISA Kit

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