Rat Defensin Beta 2 - DEFB2 ELISA Kit

Mfr.No.

E1334Ra

Sensitivity

2.61ng/L

Detection Range

5-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Defensin Beta 2 (also known as DEFb2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BD-2;BD-4;Beta-defensin 4;DEFB 4;DEFB4;Defensin, beta 4;RBD-2;RBD-4

Molecular Weight

6946

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1334Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	327.1	16.42	5
Intra-Assay	2	18	950.2	22.34	2.4
Intra-Assay	3	18	510.1	22.2	4.4

Background

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells.

Protocol

These standard curves of E1334Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.039	2.046	2.014
	2.053
600ng/L	0.922	0.93	0.898
	0.937
300ng/L	0.496	0.501	0.469
	0.505
150ng/L	0.178	0.238	0.206
	0.298
75ng/L	0.11	0.099	0.067
	0.087
0ng/L	0.038	0.032	0
	0.025

Gene ID	64389
UniProt ID	O88514

Research Area	Others

Storage	2-8°C

Reference	Imunisasi intranasal protein adhesi pili streptococcus pneumoniae untuk meningkatkan respon imun mukosa dan mencegah pneumonia melalui peningkatan s-ig Journal: Fakultas Kedokteran Universitas Jember Jl. Kalimantan 37 Jember, 2017-02-02

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112573	E1334Ra	48T	$320		Add to Cart
BHE12112573	E1334Ra	96T	$458		Add to Cart

Rat Defensin Beta 2 - DEFB2 ELISA Kit

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