Rat Cystatin C - CYS-C ELISA Kit

Mfr.No.

E0145Ra

Sensitivity

0.25ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Cystatin C (also known as CYS-C) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CST 3;CST3;Cystatin-3;Cystatin-C

Molecular Weight

15437

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0145Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.1	1.18	4.9
Intra-Assay	2	18	34	1.12	3.3
Intra-Assay	3	18	15.2	0.8	5.2

Background

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Protocol

These standard curves of E0145Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.396	2.271	2.243
	2.146
32ng/ml	1.24	1.279	1.251
	1.318
16ng/ml	0.668	0.624	0.596
	0.581
8ng/ml	0.447	0.395	0.367
	0.343
4ng/ml	0.186	0.153	0.125
	0.121
0ng/ml	0.02	0.028	0
	0.036

Gene ID	25307
UniProt ID	P14841

Research Area	Cardiovascular

Storage	2-8°C

Reference	The superiority of rosmarinic acid over vitamin e in preventing development and inhibiting progression of diabetic nephropathy in rats Journal: Asian Journal of Pharmaceutical Research. 2020, Volume : 10, Issue : 3, page 131 -139 Rosmarinic acid monotherapy is better than the combination of rosmarinic acid and telmisartan in preventing podocyte detachment and inhibiting the progression of diabetic nephropathy in rats Journal: Biologics. 2019 Aug 30;13:179-190.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111463	E0145Ra	48T	$320		Add to Cart
BHE12111463	E0145Ra	96T	$458		Add to Cart

Rat Cystatin C - CYS-C ELISA Kit

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