Rat CRP/C Reactive Protein ELISA Kit PicoKine®

Mfr.No.

EK0978

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.033	0.193	0.280	0.503	0.834	1.193	1.635	1.909

Description

Rat CRP/C Reactive Protein ELISA Kit PicoKine® (96 Tests). Quantitate Rat Crp in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-reactive protein;Crp;Ptx1;

Molecular Weight

25468 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: H20-S230

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CRP

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	225	1505	4812	235	1505	4457
Standard deviation	11.02	81.27	307.96	11.75	108.36	329.81
CV (%)	4.9%	5.4%	6.4%	5%	7.2%	7.4%

Protein Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity). .

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	225	211	199	209	211	9.27	4.3%
Sample 2	1505	1663	1572	1511	1562	63.53	4%
Sample 3	4812	4672	4766	4677	4731	59.54	1.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25419
Gene Name	CRP
UniProt ID	P48199
Protein Name	C-reactive protein
Tissue Specificity	Found in plasma.
Sequence	Belongs to the pentaxin family.

Applications	ELISA
Research Area	Apoptosis,Atherosclerosis,Cardiovascular,Chromatin Binding Proteins,Complement,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000741	EK0978	96 wells/kit	$499		Add to Cart

Rat CRP/C Reactive Protein ELISA Kit PicoKine®

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