Rat CRP/C Reactive Protein ELISA Kit PicoKine®

Mfr.No.

EK0978

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.033	0.193	0.280	0.503	0.834	1.193	1.635	1.909

Description

Rat CRP/C Reactive Protein ELISA Kit PicoKine® (96 Tests). Quantitate Rat Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-reactive protein;Crp;Ptx1;

Molecular Weight

25468 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: H20-S230

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CRP

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	225	1505	4812	235	1505	4457
Standard deviation	11.02	81.27	307.96	11.75	108.36	329.81
CV (%)	4.9%	5.4%	6.4%	5%	7.2%	7.4%

Protein Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity). .

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	225	211	199	209	211	9.27	4.3%
Sample 2	1505	1663	1572	1511	1562	63.53	4%
Sample 3	4812	4672	4766	4677	4731	59.54	1.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25419
Antigen	CRP/C
Gene Name	CRP
UniProt ID	P48199
Protein Name	C-reactive protein
Tissue Specificity	Found in plasma.
Sequence	Belongs to the pentaxin family.

Applications	ELISA
Research Area	Apoptosis,Atherosclerosis,Cardiovascular,Chromatin Binding Proteins,Complement,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000741	EK0978	96 wells/kit	$499		Add to Cart

Rat CRP/C Reactive Protein ELISA Kit PicoKine®

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