Matrices listed below were spiked with certain level of CORT and the recovery rates were calculated by comparing the measured value to the expected amount of CORT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
92
EDTA plasma(n=5)
85-103
98
heparin plasma(n=5)
85-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CORT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CORT ELISA Kit allows for the in vitro quantitative determination of CORT concentrations in serum, plasma, tissue homogenates and other biological fluids.