Rat Contactin-1 ELISA Kit PicoKine®

Mfr.No.

EK1773

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.018	0.115	0.296	0.405	0.567	0.941	1.586	2.167

Description

Rat Contactin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cntn1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Contactin-1; Neural cell surface protein F3; Cntn1

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-S1001

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Contactin-1

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	217	1103	3628	265	1145	3921
Standard deviation	9.77	62.87	166.89	18.82	79.01	250.94
CV (%)	4.5%	5.7%	4.6%	7.1%	6.9%	6.4%

Protein Function

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

Background

Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. It is mapped to 7q35. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alteatively spliced transcript variants encoding different isoforms have been described for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	217	222	227	247	228	11.38	4.9%
Sample 2	1103	1131	1250	1268	1188	71.96	6.0%
Sample 3	3628	4052	4148	3796	3906	205.70	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	117258
Gene Name	Cntn1
UniProt ID	Q63198
Tissue Specificity	Expressed by neurons, oligodendrocytes and their progenitors. Myelination regulates the expression being downregualted when neurons are in contact with Schwann cells.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001602	EK1773	96 wells/kit	$499		Add to Cart

Rat Contactin-1 ELISA Kit PicoKine®

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