Rat Ciliary Neurotrophic Factor

Mfr.No.

E0358Ra

Sensitivity

2.46ng/L

Detection Range

5-1500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Ciliary Neurotrophic Factor (also known as CNTF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Ciliary neurotrophic factor;CNTF

Molecular Weight

22854

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0358Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	126.3	6.92	5.5
Intra-Assay	2	18	281.8	13.41	4.8
Intra-Assay	3	18	103.9	5.81	5.6

Background

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Protocol

These standard curves of E0358Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.072	2.111	2.052
	2.149
400ng/L	1.715	1.702	1.643
	1.689
200ng/L	1.121	1.112	1.053
	1.102
100ng/L	0.629	0.645	0.586
	0.662
50ng/L	0.385	0.379	0.32
	0.373
0ng/L	0.065	0.059	0
	0.053

Gene ID	25707
UniProt ID	P20294

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111654	E0358Ra	48T	$320		Add to Cart
BHE12111654	E0358Ra	96T	$458		Add to Cart

Rat Ciliary Neurotrophic Factor - CNTF ELISA Kit

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