Rat Cerebral Dopamine Neurotrophic Factor

Mfr.No.

E0865Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Cerebral Dopamine Neurotrophic Factor (also known as CDNF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Arginine-rich protein mutated in early stage tumors-like 1;ARMET-like protein 1;CDNF;Cerebral dopamine neurotrophic factor;Conserved dopamine neurotrophic factor

Reactivity

Rat

Detection Range

0.05-20ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21361

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/mL

Background

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.

Gene ID	361276
UniProt ID	P0C5I0
Antigen	CDNF

Research Area

Neuroscience

Protocol

These standard curves of E0865Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.144	2.19	2.127
	2.236
6ng/mL	1.228	1.339	1.276
	1.451
3ng/mL	0.815	0.76	0.697
	0.705
1.5ng/mL	0.517	0.464	0.402
	0.412
0.75ng/mL	0.296	0.281	0.219
	0.267
0ng/mL	0.074	0.063	0
	0.052

Precision

We measured random samples of E0865Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.1	0.23	3.8
Intra-Assay	2	18	1.2	0.08	6.7
Intra-Assay	3	18	4.3	0.2	4.6

Storage	2-8°C

Reference	Effect of Withania Somnifera on the antioxidant and neurotransmitter status in sleep deprivation induced Wistar rats Journal: Bioinformation. 2020 Aug 31;16(8):631-637

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112116	E0865Ra	48T	$320		Add to Cart
BHE12112116	E0865Ra	96T	$458		Add to Cart

Rat Cerebral Dopamine Neurotrophic Factor - CDNF ELISA Kit

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