Rat Cathepsin B - CATH-B ELISA Kit

Mfr.No.

E0871Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Cathepsin B (also known as cath-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

cath-B;Cathepsin B;Cathepsin B heavy chain);Cathepsin B light chain;Cathepsin B1;RSG-2

Reactivity

Rat

Detection Range

0.2-70ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

37470

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.11ng/ml

Background

Cathepsin B belongs to a family of lysosomal cysteine proteases and plays an important role in intracellular proteolysis. In humans, cathepsin B is encoded by the CTSB gene. Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions.

Gene ID	64529
UniProt ID	P00787
Antigen	cath-B

Research Area

Others

Protocol

These standard curves of E0871Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	1.94	1.842	1.8
	1.744
20ng/ml	1.031	1.032	0.991
	1.034
10ng/ml	0.447	0.478	0.436
	0.509
5ng/ml	0.195	0.234	0.192
	0.273
2.5ng/ml	0.147	0.13	0.088
	0.112
0ng/ml	0.04	0.042	0
	0.044

Precision

We measured random samples of E0871Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.03	1	5
Intra-Assay	2	18	26.48	0.75	2.84
Intra-Assay	3	18	13.91	0.58	4.15

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112122	E0871Ra	48T	$320		Add to Cart
BHE12112122	E0871Ra	96T	$458		Add to Cart

Rat Cathepsin B - CATH-B ELISA Kit

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