Rat Catalase - CAT ELISA Kit

Mfr.No.

E0869Ra

Sensitivity

0.52ng/ml

Detection Range

1-300ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Catalase (also known as CAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CAT;Catalase

Molecular Weight

59757

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0869Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	79.4	4.57	5.8
Intra-Assay	2	18	15.7	0.83	5.3
Intra-Assay	3	18	40.2	2.99	7.4

Background

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.

Protocol

These standard curves of E0869Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	2.264	2.322	2.28
	2.381
80ng/ml	1.78	1.804	1.762
	1.828
40ng/ml	1.23	1.265	1.223
	1.301
20ng/ml	0.681	0.661	0.619
	0.64
10ng/ml	0.434	0.41	0.368
	0.387
0ng/ml	0.039	0.042	0
	0.045

Gene ID	24248
UniProt ID	P04762

Research Area	Tags & Cell Markers; Signal Transduction; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

Reference	Cannabinoid-profiled agents improve cell survival via reduction of oxidative stress and inflammation, and Nrf2 activation in a toxic model combining hyperglycemia+A?1-42 peptide in rat hippocampal neurons Journal: Neurochem Int. 2020 Nov;140:104817 Redox-active phytoconstituents ameliorate cell damage and inflammation in rat hippocampal neurons exposed to hyperglycemia+A?1-42 peptide Journal: Neurochem Int. 2021 May;145:104993 Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats Journal: J Biochem Mol Toxicol. 2023 Jul 19:e23471.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112120	E0869Ra	48T	$320		Add to Cart
BHE12112120	E0869Ra	96T	$458		Add to Cart

Rat Catalase - CAT ELISA Kit

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