Rat Calpain-2 Catalytic Subunit

Mfr.No.

E1325Ra

Sensitivity

0.021ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Calpain-2 Catalytic Subunit (also known as CAPN2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Calcium-activated neutral proteinase 2;Calpain M-type;Calpain-2 catalytic subunit;Calpain-2 large subunit;CANP 2;CAPN 2;CAPN2;M-calpain;Millimolar-calpain;

Molecular Weight

79919

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1325Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.3	0.14	3.4
Intra-Assay	2	18	1.8	0.08	4.4
Intra-Assay	3	18	2.4	0.11	4.5

Background

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.

Protocol

These standard curves of E1325Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.336	2.206	2.167
	2.076
8ng/ml	1.933	1.923	1.885
	1.914
4ng/ml	1.232	1.267	1.229
	1.302
2ng/ml	0.608	0.603	0.565
	0.598
1ng/ml	0.371	0.369	0.331
	0.368
0ng/ml	0.041	0.038	0
	0.035

Gene ID	29154
UniProt ID	Q07009

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112564	E1325Ra	48T	$320		Add to Cart
BHE12112564	E1325Ra	96T	$458		Add to Cart

Rat Calpain-2 Catalytic Subunit - CAPN2 ELISA Kit

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