Rat Calpain-2 Catalytic Subunit

Mfr.No.

E1325Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Calpain-2 Catalytic Subunit (also known as CAPN2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Calcium-activated neutral proteinase 2;Calpain M-type;Calpain-2 catalytic subunit;Calpain-2 large subunit;CANP 2;CAPN 2;CAPN2;M-calpain;Millimolar-calpain;

Reactivity

Rat

Detection Range

0.05-30ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

79919

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.

Gene ID	29154
UniProt ID	Q07009
Antigen	CAPN2

Research Area

Signal Transduction

Protocol

These standard curves of E1325Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.336	2.206	2.167
	2.076
8ng/ml	1.933	1.923	1.885
	1.914
4ng/ml	1.232	1.267	1.229
	1.302
2ng/ml	0.608	0.603	0.565
	0.598
1ng/ml	0.371	0.369	0.331
	0.368
0ng/ml	0.041	0.038	0
	0.035

Precision

We measured random samples of E1325Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.3	0.14	3.4
Intra-Assay	2	18	1.8	0.08	4.4
Intra-Assay	3	18	2.4	0.11	4.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112564	E1325Ra	48T	$320		Add to Cart
BHE12112564	E1325Ra	96T	$458		Add to Cart

Rat Calpain-2 Catalytic Subunit - CAPN2 ELISA Kit

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