Rat BMP-6 ELISA Kit PicoKine®

Mfr.No.

EK1609

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.026	0.114	0.189	0.320	0.605	1.111	1.812	2.522

Description

Rat BMP-6 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Bmp6 in bone tissue, cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 6; BMP-6; VG-1-related protein; VGR-1; Bmp6; Bmp-6, Vgr;

Immunogen

Expression system for standard: CHO; Immunogen sequence: S368-H506

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat BMP-6

Subcellular Localization

Secreted .

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum

Anticoagulant

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	286	1189	5032	301	1196	5027
Standard deviation	18.01	68.96	342.17	20.46	69.36	341.83
CV (%)	5.8%%	5.8%	6.8%	6.8%	5.8%	6.8%

Protein Function

Induces cartilage and bone formation. .

Background

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	286	295	282	248	277	17.8	6.4%
Sample 2	1189	1131	1215	1119	1163	39.8	3.4%
Sample 3	5032	5144	5474	4947	5149	200.08	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25644
Gene Name	Bmp6
UniProt ID	Q04906

Applications	ELISA
Research Area	Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors/Hormones,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001281	EK1609	96 wells/kit	$499		Add to Cart

Rat BMP-6 ELISA Kit PicoKine®

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