Rat Betaine--homocysteine S-methyltransferase 1

Mfr.No.

E1886Ra

Alternative Names

Betaine--homocysteine S-methyltransferase 1;BHMT

Reactivity

Rat

Detection Range

20-4500ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

44976

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.85ng/L

Background

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Gene ID	81508
UniProt ID	O09171
Antigen	BHMT

Research Area

Signal Transduction

Protocol

These standard curves of E1886Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.067	2.164	2.129
	2.26
1200ng/L	1.28	1.282	1.247
	1.283
600ng/L	0.785	0.782	0.747
	0.779
300ng/L	0.398	0.394	0.36
	0.391
150ng/L	0.17	0.188	0.154
	0.207
0ng/L	0.035	0.035	0
	0.035

Precision

We measured random samples of E1886Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1024.8	63.18	6.17
Intra-Assay	2	18	1130.13	37.39	3.31
Intra-Assay	3	18	391.27	25.24	6.45

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113085	E1886Ra	48T	$320		Add to Cart
BHE12113085	E1886Ra	96T	$458		Add to Cart

Rat Betaine--homocysteine S-methyltransferase 1 - BHMT ELISA Kit

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