Rat beta IG-H3/TGFBI ELISA Kit PicoKine®

Mfr.No.

EK1571

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.010	0.031	0.064	0.137	0.376	0.922	1.352	1.879

Description

Rat beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests). Quantitate Rat TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor-beta-induced protein ig-h3;Beta ig-h3;Kerato-epithelin;RGD-containing collagen-associated protein;RGD-CAP;TGFBI;BIGH3;

Molecular Weight

74681 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-G685

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat beta IG-H3

Subcellular Localization

Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	308	1355	5623	336	1296	5160
Standard deviation	20.63	73.17	314.88	24.19	84.24	309.6
CV (%)	6.7%	5.4%	5.6%	7.2%	6.5%	6%

Protein Function

Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Background

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	308	274	298	308	297	13.89	4.6%
Sample 2	1355	1497	1581	1353	1446	97.15	6.7%
Sample 3	5623	6215	5716	5905	5864	226.3	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	116487
Antigen	IG-H3/TGFBI
Gene Name	TGFBI
UniProt ID	Q15582
Protein Name	Transforming growth factor-beta-induced protein ig-h3
Tissue Specificity	Highly expressed in the corneal epithelium. .

Applications	ELISA
Research Area	Collagen,Cytoskeleton/ECM,Developmental Biology,ECM Proteins,Extracellular Matrix,Growth Factors/Hormones,Neuroscience,Organogenesis,Sensory System,Signal Transduction,Structures,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001130	EK1571	96 wells/kit	$499		Add to Cart

Rat beta IG-H3/TGFBI ELISA Kit PicoKine®

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