Matrices listed below were spiked with certain level of beta-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of beta-CTx in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-94
91
EDTA plasma(n=5)
90-101
96
heparin plasma(n=5)
87-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
beta-CTx ELISA Kit allows for the in vitro quantitative determination of beta-CTx concentrations in serum, plasma, tissue homogenates and other biological fluids.