Rat AP13(Apelin 13) ELISA Kit

Mfr.No.

ELK10782

Sensitivity

0.23 ng/mL

Detection Range

0.78-50 ng/mL

Assay Duration

Assay Type

Competitive Inhibition

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AP13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	82-96%	87-103%	85-97%
EDTA Plasma(n=5)	88-97%	86-92%	81-93%	93-104%
Heparin Plasma(n=5)	87-93%	93-105%	85-99%	90-99%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
50.00	0.217
25.00	0.479
12.50	0.786
6.25	0.967
3.13	1.258
1.57	1.596
0.79	1.877
0.00	2.219

Sample Type

Serum, Plasma and other biological fluids.

Limit Of Detection

50 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant AP13 and the recovery rates were calculated by comparing the measured value to the expected amount of AP13 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA Plasma(n=5)	85-97%	91%
Heparin Plasma(n=5)	81-93%	99%

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209963	ELK10782	48T	$320		Add to Cart
BHE15209963	ELK10782	96T	$458		Add to Cart

Rat AP13(Apelin 13) ELISA Kit

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