Matrices listed below were spiked with certain level of Amelx and the recovery rates were calculated by comparing the measured value to the expected amount of Amelx in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
93
EDTA plasma(n=5)
86-99
92
heparin plasma(n=5)
93-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amelx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Amelx ELISA Kit allows for the in vitro quantitative determination of Amelx concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Amelx(Amelogenin, X isoform) ELISA Kit