Rat Alanine--glyoxylate Aminotransferase 2 - Mitochondrial

Mfr.No.

E1853Ra

Sensitivity

7.19ng/L

Detection Range

15-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

(R-3-amino-2-methylpropionate--pyruvate transaminase;AGT 2;AGXT 2;AGXT2;Alanine--glyoxylate aminotransferase 2, mitochondrial;Beta-ALAAT II;Beta-alanine-pyruvate aminotransferase;D-AIBAT

Molecular Weight

57201

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1853Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	947.3	30.19	3.2
Intra-Assay	2	18	247.8	12.74	5.1
Intra-Assay	3	18	419.6	23.93	5.7

Background

Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) Synthase, and this activity provides mechanism through which the kidney regulates blood pressure (By similarity).

Protocol

These standard curves of E1853Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.616	2.652	2.617
	2.689
800ng/L	1.33	1.39	1.354
	1.45
400ng/L	0.8	0.866	0.83
	0.932
200ng/L	0.452	0.468	0.432
	0.484
100ng/L	0.245	0.238	0.202
	0.231
0ng/L	0.032	0.036	0
	0.04

Gene ID	83784
UniProt ID	Q64565

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113052	E1853Ra	48T	$320		Add to Cart
BHE12113052	E1853Ra	96T	$458		Add to Cart

Rat Alanine--glyoxylate Aminotransferase 2 - Mitochondrial - AGXT2 ELISA Kit

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