Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 4

Mfr.No.

E1722Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 4 (also known as ADAMTS4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 4;ADAMTS 4;ADAM-TS 4;ADAMTS4;ADAMTS-4;ADAM-TS4;Aggrecanase-1;Fragment

Reactivity

Rat

Detection Range

5-300ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

68385

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.02ng/ml

Background

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Gene ID	66015
UniProt ID	Q9ESP7
Antigen	ADAMTS4

Research Area

Others

Protocol

These standard curves of E1722Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	2.313	2.262	2.209
	2.211
80ng/ml	1.695	1.65	1.596
	1.606
40ng/ml	1.084	1.059	1.005
	1.034
20ng/ml	0.701	0.686	0.633
	0.672
10ng/ml	0.455	0.466	0.412
	0.477
0ng/ml	0.044	0.054	0
	0.064

Precision

We measured random samples of E1722Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.8	1.33	7.4
Intra-Assay	2	18	28.4	1.91	6.7
Intra-Assay	3	18	59.5	2.68	4.5

Storage	2-8°C

Reference	The Effect of Mesenchymal Stem Cell Whartons Jelly on ADAMTS-4 and iNOS Levels in Osteoarthritis Rat Model Journal: Open Access Maced J Med Sci. 2019 Apr 29;7(8):1270-1275.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112928	E1722Ra	48T	$320		Add to Cart
BHE12112928	E1722Ra	96T	$458		Add to Cart

Rat Adisintegrin and Metalloproteinase with Thrombospondin Motifs 4 - ADAMTS4 ELISA Kit

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