Rat 14-3-3 Protein Gamma - YWHAG ELISA Kit

Mfr.No.

E1831Ra

Sensitivity

0.048ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

14-3-3 protein gamma;N-terminally processed;YWHAG

Molecular Weight

28303

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1831Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.5	0.69	4.7
Intra-Assay	2	18	7.6	0.42	5.6
Intra-Assay	3	18	4.9	0.29	5.9

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Protocol

These standard curves of E1831Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.039	2.088	2.048
	2.137
12ng/mL	1.473	1.426	1.386
	1.379
6ng/mL	0.781	0.802	0.761
	0.822
3ng/mL	0.402	0.432	0.391
	0.461
1.5ng/mL	0.217	0.241	0.201
	0.265
0ng/mL	0.036	0.04	0
	0.044

Gene ID	56010
UniProt ID	P61983

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113030	E1831Ra	48T	$320		Add to Cart
BHE12113030	E1831Ra	96T	$458		Add to Cart

Rat 14-3-3 Protein Gamma - YWHAG ELISA Kit

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