Rat 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Gamma-1

Mfr.No.

E1833Ra

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-gamma-1;PLCG1;PLC-gamma-1;PPLCA

Reactivity

Rat

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

148548

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.26ng/L

Background

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:7531435). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:7531435). Plays a role in actin reorganization and cell migration (By similarity).

Gene ID	25738
UniProt ID	P10686
Antigen	PLCG1

Research Area

Cardiovascular

Protocol

These standard curves of E1833Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.357	2.201	2.162
	2.044
800ng/L	1.541	1.568	1.529
	1.595
400ng/L	1.039	1.004	0.965
	0.969
200ng/L	0.721	0.725	0.687
	0.729
100ng/L	0.441	0.467	0.429
	0.494
0ng/L	0.028	0.039	0
	0.05

Precision

We measured random samples of E1833Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	568.66	24.28	4.27
Intra-Assay	2	18	229.43	17.2	7.5
Intra-Assay	3	18	189.55	15.82	8.34

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113032	E1833Ra	48T	$320		Add to Cart
BHE12113032	E1833Ra	96T	$458		Add to Cart

Rat 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Gamma-1 - PLCG1 ELISA Kit

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