Rabbit TGF Beta 1 ELISA Kit PicoKine®

Mfr.No.

EK0513-RB

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rabbit

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.039	0.108	0.186	0.321	0.599	1.017	1.586	2.000

Description

Rabbit TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit TGFB1 in cell culture supeatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB;

Molecular Weight

44341 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: A279-S390

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Assay Procedure Summary

Activate the sample

Cell culture supeatants, urine: add activating reagent pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

Serum, plasma(EDTA): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant TGF? 1.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

EDTA

Activating Reagent

TGF beta 1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	135	264	521	152	270	543
Standard deviation	3.64	13.73	18.8	7.6	17.3	32.04
CV (%)	4.4%	5.2%	3.6%	5%	6.4%	5.9%

Protein Function

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. .

Background

Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100008645
Gene Name	TGFB1
UniProt ID	P01137
Protein Name	Transforming growth factor beta-1
Tissue Specificity	Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. .
Sequence	Belongs to the TGF-beta family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Co-Factors,Cofactors, Vitamins/Minerals,Developmental Biology,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Metabolism,Metabolism Processes,Organogenesis,Pathways and Processes,Response To Hypoxia,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001223	EK0513-RB	96 wells/kit	$399		Add to Cart

Rabbit TGF Beta 1 ELISA Kit PicoKine®

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