Rabbit Neurotrophin-3 ELISA Kit PicoKine®

Mfr.No.

EK0472-RB

Sensitivity

<2 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rabbit

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.073	0.148	0.277	0.561	0.870	1.686	2.529

Description

Rabbit Neurotrophin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit NTF3 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;NTF3;

Molecular Weight

29355 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y139-T257

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	93	357	657	146	375	732
Standard deviation	4	17.5	34.2	7.88	22.9	47.58
CV (%)	4.3	4.9	5.2	5.4	6.1	6.5

Protein Function

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Background

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100345073
Antigen	Neurotrophin-3
Gene Name	NTF3
UniProt ID	P20783
Protein Name	Neurotrophin-3
Tissue Specificity	Brain and peripheral tissues.

Applications	ELISA
Research Area	Growth and Development,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001221	EK0472-RB	96 wells/kit	$450		Add to Cart

Rabbit Neurotrophin-3 ELISA Kit PicoKine®

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