Rabbit Neurotrophin-3 ELISA Kit PicoKine®

Mfr.No.

EK0472-RB

Sensitivity

<2 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rabbit

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.073	0.148	0.277	0.561	0.870	1.686	2.529

Description

Rabbit Neurotrophin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit NTF3 in cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;NTF3;

Molecular Weight

29355 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y139-T257

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	93	357	657	146	375	732
Standard deviation	4	17.5	34.2	7.88	22.9	47.58
CV (%)	4.3	4.9	5.2	5.4	6.1	6.5

Protein Function

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Background

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100345073
Gene Name	NTF3
UniProt ID	P20783
Protein Name	Neurotrophin-3
Tissue Specificity	Brain and peripheral tissues.

Applications	ELISA
Research Area	Growth and Development,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001221	EK0472-RB	96 wells/kit	$450		Add to Cart

Rabbit Neurotrophin-3 ELISA Kit PicoKine®

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