Rabbit Immunoglobulin A,IgA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E06946Rb

Alternative Names

Immunoglobulin A,IgA

Reactivity

Rabbit

Detection Range

12 ng/mL-3000 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of horse TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	83-98
EDTA plasma (n=4)	94	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-97
1:2	Average %	92
	Range %	84-99
1:4	Average %	95
	Range %	92-103
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Sensitivity

3 ng/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin A,IgA

Applications

ELISA

Research Area

IgA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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