Rabbit Collagen Type I,Col I ELISA Kit

Mfr.No.

CSB-E06858Rb

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I, Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I

Recovery

The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	86-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-87
1:2	Average %	98
	Range %	94-103
1:4	Average %	88
	Range %	84-92
1:8	Average %	108
	Range %	104-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.049	2.102	2.076	1.960
1000	1.604	1.586	1.595	1.479
500	1.080	1.102	1.091	0.975
250	0.651	0.672	0.662	0.546
125	0.424	0.402	0.413	0.297
62.5	0.262	0.271	0.267	0.151
31.25	0.206	0.199	0.203	0.087
0	0.117	0.115	0.116	?

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Col I

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507326	CSB-E06858Rb	96T	$856	Add to Cart
BHE10507326	CSB-E06858Rb	5x96T	$4108.80	Add to Cart
BHE10507326	CSB-E06858Rb	10x96T	$8132	Add to Cart

Rabbit Collagen Type I,Col I ELISA Kit

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