Porcine Insulin - INS ELISA Kit

Mfr.No.

E0283Po

Sensitivity

1.43uIU/ml

Detection Range

3-900uIU/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

INS;Insulin A chain;Insulin Insulin B chain

Molecular Weight

11672

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0283Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	270.3	8.61	3.2
Intra-Assay	2	18	45.9	2.41	5.3
Intra-Assay	3	18	138.8	7.63	5.5

Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Protocol

These standard curves of E0283Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480uIU/ml	2.237	2.149	2.105
	2.06
240uIU/ml	1.29	1.294	1.251
	1.299
120uIU/ml	0.807	0.809	0.765
	0.81
60uIU/ml	0.493	0.453	0.409
	0.413
30uIU/ml	0.208	0.193	0.149
	0.179
0uIU/ml	0.025	0.044	0
	0.062

Gene ID	397415
UniProt ID	P01315

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115240	E0283Po	48T	$320		Add to Cart
BHE12115240	E0283Po	96T	$458		Add to Cart

Porcine Insulin - INS ELISA Kit

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