Porcine Hydroxyacyl-coenzyme A Dehydrogenase - Mitochondrial

Mfr.No.

E0572Po

Alternative Names

HAD;HADH;HADHSC;HCDH;Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial;L-3-hydroxyacyl CoA dehydrogenase;Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase;Short-chain 3-hydroxyacyl-CoA dehydrogenase

Reactivity

Porcine

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34161

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.98ng/L

Background

Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed:9593854, PubMed:2817332). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity).

Gene ID	397604
UniProt ID	P00348
Antigen	HADH

Research Area

Signal Transduction; Cancer; Metabolism

Protocol

These standard curves of E0572Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.788	1.831	1.777
	1.875
1200ng/L	1.09	1.088	1.034
	1.087
600ng/L	0.415	0.458	0.404
	0.5
300ng/L	0.281	0.27	0.216
	0.259
150ng/L	0.126	0.122	0.068
	0.117
0ng/L	0.051	0.054	0
	0.057

Precision

We measured random samples of E0572Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1562.74	49.56	3.17
Intra-Assay	2	18	553.33	23.84	4.31
Intra-Assay	3	18	1401.54	66.96	4.78

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110945	E0572Po	48T	$320		Add to Cart
BHE12110945	E0572Po	96T	$458		Add to Cart

Porcine Hydroxyacyl-coenzyme A Dehydrogenase - Mitochondrial - HADH ELISA Kit

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