Porcine Epithelial Growth Factor

Mfr.No.

E0556Po

Alternative Names

EGF;Epidermal growth factor;Pro-epidermal growth factor

Reactivity

Porcine

Detection Range

200-20000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

133506

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

98.23ng/L

Background

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium Channel TRPM6 (By similarity).

Gene ID	397083
UniProt ID	Q00968
Antigen	EGF

Research Area

Signal Transduction; Cell Biology; Cancer

Protocol

These standard curves of E0556Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12000ng/L	1.88	1.895	1.852
	1.91
6000ng/L	1.104	1.106	1.063
	1.109
3000ng/L	0.536	0.578	0.535
	0.62
1500ng/L	0.285	0.247	0.205
	0.21
750ng/L	0.068	0.069	0.027
	0.07
0ng/L	0.061	0.043	0
	0.024

Precision

We measured random samples of E0556Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3124.3	134.99	4.3
Intra-Assay	2	18	11360.6	532.31	4.7
Intra-Assay	3	18	6193.9	313.35	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110929	E0556Po	48T	$320		Add to Cart
BHE12110929	E0556Po	96T	$458		Add to Cart

Porcine Epithelial Growth Factor - EGF ELISA Kit

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